Quantum yield determination by low-intensity Fluorescence Correlation Spectroscopy (liFCS)
نویسندگان
چکیده
Fluorescence correlation spectroscopy (FCS) is a method based on the correlation analysis of the temporal behaviour of fluorescence intensity fluctuations. It is commonly used to determine diffusion coefficients and concentrations of samples in the (sub)nano-molar concentration regime. Another quantity made accessible by FCS is the mean number of detected fluorescence photons per molecule and time, also referred to as molecular brightness (MB).
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